Use of reference libraries and hybridisation fingerprinting for relational genome analysis

FEBS Letters

Hoheisel, Jörg D.¹  Lehrach, Hans¹ 

[1] Genome Analysis Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX, UK

关键词: Genome analysis;    Hybridisation;    Reference library;    Mapping;    Sequencing;   

DOI  :  10.1016/0014-5793(93)81426-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The concept of relational genome analysis by hybridisation has been developed into a working system. Various genomic and cDNA libraries have been generated and are distributed via a reference system. Analysis procedures have been tested successfully in the mapping of the entire Schizosaccharomyces pombe genome. In another test-case for their refinement, analyses on the Drosophila genome are well under way. Human and mouse libraries are being studied on all levels, from generating YAC maps to partially sequencing representative cDNA libraries. The automation of the involved processes and the development of improved image detection and analysis are well advanced.

【 授权许可】

Unknown