Engineering type 1 copper sites in proteins

FEBS Letters

Canters, G.W.¹  Gilardi, G.¹ 

[1] Gorlaeus Laboratories, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

关键词: Blue copper;    Site-directed mutagenesis;    Electron transfer;    Redox potential;    Type 2;    Azurin;   

DOI  :  10.1016/0014-5793(93)81410-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The use of site-directed mutagenesis methods has revolutionalized the study of the so-called type 1 and type 2 copper sites in proteins. In particular our understanding of the relation between the structure, and the mechanistic and spectroscopic features of these sites is benefitting from the application of these techniques. Recent progress in the field is reviewed with emphasis on the study of type 1 sites. Topics covered comprise the characteristics of the natural type 1 and type 2 sites, the genetics of blue copper proteins, the modification of Cu sites, the spectroscopy of natural and engineered type 1 and type 2 sites, the effect of mutations on midpoint potentials and the mechanism of electron transfer as carried out by the blue copper proteins.

【 授权许可】

Unknown