Production of monoclonal antibodies that inhibit ADP‐ribosylation of small GTP‐binding proteins catalyzed by Clostridium botulinum ADP‐ribosyltransferase C3

FEBS Letters

Sekine, Nobuyuki¹  Katada, Toshiaki¹  Toratani, Satoshi²  Yokosawa, Hideyoshi² 

[1] Department of Life Science, Tokyo Institute of Technology, Yokohama 227, Japan;Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: ADP-ribosylation;    GTP-binding protein;    Monoclonal antibody;    Clostridium botulinum;    C3;   

DOI  :  10.1016/0014-5793(93)80150-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four monoclonal antibodies that inhibited ADP-ribosylation of 23 kDa protein(s) of ascidian eggs catalyzed by Clostridium botulinum ADP-ribosyltransferase C3 were produced. They also inhibited C3-catalyzed ADP-ribosylation of the 24 kDa protein of rat liver cytosol. By the immunoprecipitation technique, it was found that they recognized small GTP-binding proteins of ascidian eggs and mammalian brains, but did not interact with the rat brain activator of the ADP-ribosyltransferase reaction. The antibody can also immunoprecipitate recombinant Rho A irrespective as to whether the Rho A is the GDP-bound form or the GTPrS-bound form. Thus the antibodies are novel and useful tools in analyzing the physiological roles of the Rho family of GTP-binding proteins.

【 授权许可】

Unknown