FEBS Letters | |
Neurotrophin induced cAMP and IP3 responses in PC12 cells | |
Rylett, Jane1  Breer, Heinz2  Beck, Anja2  Knipper, Marlies1  | |
[1] Department of Physiology, University of Western Ontario, London, Ont. N6A 5C1, Canada;University of Hohenheim, Institute of Zoophysiology, Garbenstraße 30, 7000 Stuttgart 70, Germany | |
关键词: NGF; BDNF; cAMP; IP3; p75NGFR; trkNGFR; cAMP; 3'5'-cyclic adenosine monophosphate; EDTA; ethylene-diaminetetraacetate; EGTA; ethyleneglycol bis(aminoethylether)tetraacetate; IP3 inositoltrisphosphate; NGF; nerve growth factor; BDNF; brain-derived neurotrophin factor; p75NGFR; low-affinity NGF receptor; trkNGFR; high-affinity NGF receptor; | |
DOI : 10.1016/0014-5793(93)81382-A | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
NGF and BDNF elevate cAMP and IP3 levels in membranes of PC12 cells within a subsecond time period. The cAM P formation induced by NGF and BDNF pretreatment for 2 s was reduced by GDP-β-S and PTX, but not the trkNGFR inhibitor K 252a. NGF, but not BDNF, induced IP3 formation. IP3 formation was reduced by K 252a, but not by GDP-β-S and PTX. Using p75NGFR expressing, but trkNGFR-deficient PCNA cell membranes, NGF and BDNF induced cAMP formation, but not IP3 formation. We suggest that NGF and BDNF induced cAMP formation is mediated via a p75NGFR/G-protein mediated mechanism, and IP3 formation via a K 252a sensitive pathway.
【 授权许可】
Unknown
【 预 览 】
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