Neurotrophin induced cAMP and IP3 responses in PC12 cells

FEBS Letters

Rylett, Jane¹  Breer, Heinz²  Beck, Anja²  Knipper, Marlies¹ 

[1] Department of Physiology, University of Western Ontario, London, Ont. N6A 5C1, Canada;University of Hohenheim, Institute of Zoophysiology, Garbenstraße 30, 7000 Stuttgart 70, Germany

关键词: NGF;    BDNF;    cAMP;    IP3;    p75NGFR;    trkNGFR;    cAMP;    3'5'-cyclic adenosine monophosphate;    EDTA;    ethylene-diaminetetraacetate;    EGTA;    ethyleneglycol bis(aminoethylether)tetraacetate;    IP3 inositoltrisphosphate;    NGF;    nerve growth factor;    BDNF;    brain-derived neurotrophin factor;    p75NGFR;    low-affinity NGF receptor;    trkNGFR;    high-affinity NGF receptor;   

DOI  :  10.1016/0014-5793(93)81382-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NGF and BDNF elevate cAMP and IP₃ levels in membranes of PC12 cells within a subsecond time period. The cAM P formation induced by NGF and BDNF pretreatment for 2 s was reduced by GDP-β-S and PTX, but not the trk^NGFR inhibitor K 252a. NGF, but not BDNF, induced IP₃ formation. IP₃ formation was reduced by K 252a, but not by GDP-β-S and PTX. Using p75^NGFR expressing, but trk^NGFR-deficient PCNA cell membranes, NGF and BDNF induced cAMP formation, but not IP₃ formation. We suggest that NGF and BDNF induced cAMP formation is mediated via a p75^NGFR/G-protein mediated mechanism, and IP₃ formation via a K 252a sensitive pathway.

【 授权许可】

Unknown