Calcium dependent activation of the NF‐AT transcription factor by p59fyn

FEBS Letters

Telford, John L.¹  Baldari, Cosima T.²  Heguy, Adriana¹ 

[1] Immunobiology Research Institute Siena, Via Fiorentina 1, 53100 Siena, Italy;Department of Evolutionary Biology, University of Siena, Via Mattioli 4, 53100 Siena, Italy

关键词: T-cell activation;    p59fyn;    Tyrosine kinase;    Calcium;    PKC;    protein kinase C;    TCR;    T-cell antigen receptor;    CsA;    cyclosporin A;    CAT;    chloramphenicol acetyltransferase;    PMA;    phorbol myristate acetate;    IL-2;    interleukin-2;   

DOI  :  10.1016/0014-5793(93)81346-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A reporter gene under the control of a T-cell antigen receptor response element was activated in Jurkat cells by antigen receptor triggering or by a combination of phorbol myristate acetate, which activates protein kinase C, and a calcium ionophore. Both these signals were necessary for expression of the reporter gene. When co-transfected with a construct capable of overexpressing the tyrosine kinase p59fyn, the reporter gene was activated by PMA alone. Thus p59fyn could replace the calcium ionophore but not activation of protein kinase C. The activation by p59fyn plus PMA was blocked by EGTA and by the immunosuppressant drug cyclosporin A.

【 授权许可】

Unknown