ICAM-1 depletion in the center of immunological synapses is important for calcium releasing in T-cells

Journal of Innovative Optical Health Sciences

关键词: T-cell activation;    immunological synapse;    ICAM-1;    calcium signaling;   

DOI  :  10.1142/S1793545817500158

学科分类：医学（综合）

来源: World Scientific Publishing Co. Pte. Ltd.

【 摘 要 】

T-cell activation requires the formation of the immunological synapse (IS) between a T-cell and an antigen-presenting cell (APC) to control the development of the adaptive immune response. However, calcium release, an initial signal of T-cell activation, has been found to occur before IS formation. The mechanism for triggering the calcium signaling and relationship between calcium release and IS formation remains unclear. Herein, using live-cell imaging, we found that intercellular adhesion molecule 1 (ICAM-1), an essential molecule for IS formation, accumulated and then was depleted at the center of the synapse before complete IS formation. During the process of ICAM-1 depletion, calcium was released. If ICAM-1 failed to be depleted from the center of the synapse, the sustained calcium signaling could not be induced. Moreover, depletion of ICAM-1 in ISs preferentially occurred with the contact of antigen-specific T-cells and dendritic cells (DCs). Blocking the binding of ICAM-1 and lymphocyte function-as...

【 授权许可】

Unknown