Immunocytochemical analyses of annexin V (CaBP33) in a human‐derived glioma cell line

FEBS Letters

Sorci, Guglielmo¹  Giambanco, Ileana¹  Spreca, Antonio¹  Bocchini, Virginia²  Rambotti, Maria Grazia¹  Antonioli, Sabrina¹  Donato, Rosario¹ 

[1] Sections of Anatomy, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, C.P. 81 Succ. 3, 06100 Perugia, Italy;Cellular and Molecular Biology, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, C.P. 81 Succ. 3, 06100 Perugia, Italy

关键词: Annexin V (CaBP33);    Glioma cell;    Immunocytochemistry;    Electron microscope;    Cell growth;   

DOI  :  10.1016/0014-5793(93)81445-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The subcellular distribution of annexin V, a calcium-dependent phospholipid- and membrane-binding protein, in a human-derived cell line, GL15, was investigated by immunocytochemistry at light and electron microscope levels. Annexin V was found diffusely in the cytoplasm and associated with plasma membranes, membranes delimiting cytoplasmic vacuoles, membranes of the endoplasmic reticulum, and filamentous structures the identity of which remains to be established. By immunocytochemistry at the light microscope level and immunochemistry, the expression of annexin V in these cells was found to depend on cellular growth state, being maximal soon after plating and progressively declining thereafter. However, re-expression of annexin V was observed whenever cell proliferation slowed down or arrested. These findings suggest that annexin V in glioma cells is mostly expressed in connection with cell differentiation. Also, the present ultrastructural data suggest that plasma membranes, membranes of the endoplasmic reticulum and the cytoskeleton are prominent sites of action of annexin V in vivo, thus lending support to the possibility that this protein might have a role in the regulation of cytoskeleton elements and/or of the structural organization of membranes.

【 授权许可】

Unknown