Increased expression of F1ATP synthase subunits in yeast strains carrying point mutations which destabilise the β subunit

FEBS Letters

Zak, Radovan²  Aschenbrenner, Marketa²  Mueller, David M.¹  Wiesner, Rudolf J.² 

[1] Departments of Biological Chemistry and Structure, University of Health Sciences, The Chicago Medical School, North Chicago, IL, USA;Department of Medicine, The University of Chicago, Chicago, IL, USA

关键词: Mitochondrion;    F1;    ATP synthase;    Gene expression;    Intracellular signaling;    Regulation of transcription;    Point mutation;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(93)81441-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In yeast strains (S. cerevisiae) carrying a point mutation of the ATP2 gene, which destabilizes the β subunit of F₁, ATP synthase in vitro, the growth rate was reduced significantly, demonstrating that the mutation is also deleterious in vivo. Immunoblots showed that levels of the mutated β, but also of the wild-type α subunit were increased in the mutated strains, together with levels of the corresponding mRNAs (approximately 1.6-fold). Northern analysis showed that this was due to both the appearance of new transcript species as well as upregulation of the cognate transcripts, strongly indicating that the increase was probably due to activation of transcription. Levels of other mitochondrial proteins, e.g. cytochrome c oxidase, were unaffected. We conclude that a specific signal communicates the actual performance of the ATP synthase inside the mitochondria to the nuclear genes encoding its subunits.

【 授权许可】

Unknown