A 31P NMR study of the interaction of the antitumor active metallocene Cp2MoCl2 with calf thymus DNA

FEBS Letters

Field, Leslie D.¹  Harding, Margaret M.¹  Harden, Grahame J.¹ 

[1] Department of Organic Chemistry, University of Sydney, Sydney, N.S.W. 2006, Australia

关键词: Metallocene;    Calf thymus DNA;    Antitumor;    31P NMR;   

DOI  :  10.1016/0014-5793(93)81588-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of sonicated calf thymus DNA with the antitumor active metallocene Cp₂MoCl₂ afforded a metallocene-DNA complex which was characterized by ³¹P NMR spectroscopy. In addition to the resonance for the phosphate backbone (δ −1.6), the spectrum contained 2 signals assigned to a phosphate bound Mo—DNA complex(es) (δ 37.2, 36.5) and a broad signal at δ 6.2 ppm. This result suggests that covalent attachment of the metallocene Cp₂MoCl₂ occurs via phosphate(O) coordination and is accompanied by local distortion of the DNA backbone. This result supports recent ICP studies with Cp₂TiCl₂ that have DNA detected DNA—metallocene adducts.

【 授权许可】

Unknown