Anti‐CD9 monoclonal antibody elicits staurosporine inhibitable phosphatidylinositol 4,5‐bisphosphate hydrolysis, phosphatidylinositol 3,4‐bisphosphate synthesis, and protein‐tyrosine phosphorylation in human platelets

FEBS Letters

Yatomi, Yutaka¹  Satoh, Kaneo¹  Kume, Shoji¹  Ozaki, Yukio¹ 

[1] Department of Laboratory Medicine, Yamanashi Medical College, Yamanashi, Japan

关键词: CD9;    Staurosporine;    Inositol phospholipid;    Phosphatidylinositol 3-kinase;    Protein-tyrosine phosphorylation;    Human platelet;    FcγRII;    immunoglobulin G Fc receptor type II;    PLA2;    phospholipase A2;    PLC;    phospholipase C;    G protein;    guanine nucleotide-binding protein;    PtdIns;    PtdIns(4;    5)P2;    and PtdIns(3;    4)P2;    phosphatidylinositol and its specified monoester phosphate derivatives;    PA;    phosphatidic acid;    ASA;    acetylsalicylic acid;    HPLC;    high-performance liquid chromatography;    SAX;    strong anion exchange;    GroPIns;    Gro(4;    5)P2;    and Gro(3;    4)P2;    glycerophosphoinositol and its specified monoester phosphate derivatives;   

DOI  :  10.1016/0014-5793(93)81587-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphoinositide metabolism elicited by anti-CD9 monoclonal antibody, a well-characterized platelet activator, was studied using acetylsalicylic acid-treated human platelets. TP82, which is an anti-CD9 monoclonal antibody, induced classical phosphatidylinositol 4,5-bisphosphate hydrolysis, as monitored by intracellular Ca²⁺ mobilization and phosphatidic acid production, and synthesis of phosphatidylinositol 3,4-bisphosphate, which is a major component of newly-described 3-phosphorylated inositol phospholipids produced during platelet activation. These changes were severely inhibited by 1 μM staurosporine, a potent, though non-selective, protein kinase inhibitor, which also abolished TP82 induction of tyrosine phosphorylation of multiple platelet proteins. Protein-tyrosine phosphorylation appears necessary to initiate both the classical phosphoinositide turnover and synthesis of the newly-described 3-phosphorylated inositol phospholipids in anti-CD9 monoclonal antibody-induced platelet activation.

【 授权许可】

Unknown