Phase transitions of Acholeplasma laidlawii membranes The involvement of Mg2+‐ATPase in the C transition

FEBS Letters

Hu, Liao-Yuan¹  Hwang, Fen¹  Chen, Jian-Wen¹ 

[1] Institute of Biophysics, Academia Sinica, Beijing 100101, China

关键词: A. laidlawii membrane;    Mg2+-ATPase;    DSC;   

DOI  :  10.1016/0014-5793(93)81581-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Highly sensitive differential scanning calorimetry has been employed to study the phase transitions of A. laidlawii membrane. The DSC curves obtained show five distinct transitions between 20 and 80°C which contain a reversible lipid thermotropic transition at about 37°C and four irreversible denaturation transitions of the membrane proteins occurred at about 44°C, 52°C, 62°C and 67°C, respectively. Total enthalpy of the thermal denaturation of membrane proteins is 3.4 ± 0.5 cal/g. Further study of A. laidlawii membrane preparations by means of thermal gel analysis and enzyme acitvity measurements at various temperatures provided information that the third peak (C transition) of the DSC curve involved primarity with Mg²⁺-ATPase on A. laidlawii membranes.

【 授权许可】

Unknown