An electrogenic proton pump in plasma membranes from the cellular slime mould Dictyostelium discoideum

FEBS Letters

Strandmann, Ralph Pogge-von¹  Dufour, Jean-Pierre¹  Kay, Robert R.¹ 

[1] Imperial Cancer Research Fund, Mill Hill Laboratories, Burtonhole Lane, London NW7 1AD, England

关键词: Dictyostelium discoidem;    Proton pump;    Mg2+-ATPase;    Plasma membrane;    ACMA;    9-amino-6-chloro-2-methoxy acridine;    CCCP;    carbonyl cyanide m-chlorophenyl-hydrazone;    DES;    diethylstilbestrol;    DIF;    differentiation inducing factor;    I 50;    inhibitor concentration giving 50% inhibition;    PMSF;    phenylmethylsulphonyl fluoride;   

DOI  :  10.1016/0014-5793(84)80781-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plants and fungi possess an outwardly directed plasma membrane proton pump that may regulate intracellular pH. We provide the first demonstration that amoebae of the slime mould Dictyostelium discoideum also possess a similar proton pump. It can be assayed either as an ATPase activity in highly purified plasma membranes or as a proton pump, after solubilization and reconstruction into liposomes. The pump is inhibited by vanadate, diethylstilbestrol (DES) and miconazole but not by azide or ouabain. The proton pump described here may represent the target for the action of DES and miconazole, both of which have previously been shown to induce stalk cell formation during the in vitro development of Dictyostelium.

【 授权许可】

Unknown