Expression of a novel human homeobox‐containing gene that maps to chromosome 7q36.1 in hematopoietic cells

FEBS Letters

Matsui, Toshikazu¹  Kurosawa, Yoshikazu¹  Wakita, Machiko²  Hirano, Masami²  Hirai, Momoki³ 

[1] Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-11, Japan;Department of Internal Medicine, Fujita Health University, Toyoake, Aichi 470-11, Japan;Department of Anthropology, Faculty of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Polymerase chain reaction;    Fluorescence in situ hybridization;    Hematopoiesis;    FISH;    fluorescence in situ hybridization;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(93)81564-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A homeobox is a DNA sequence of 180 base pairs that encodes a DNA-binding domain known as a homeodomain. The polymerase chain reaction (PCR) has been used to prepare probes of homeobox-containing genes. We cloned and sequenced the amplified products of PCR that was performed with human genomic DNA and two primers that correspond to well-conserved regions in homeoboxes. Fifteen kinds of homeobox gene were identified and 13 of them were assigned to HOX genes that have already been reported. Two others represented novel homeobox genes and one of them, GBX1, was mapped to chromosome 7q36.1 by fluorescence in situ hybridization. Northern hybridization of mRNA for various kinds of hematopoietic cell showed that the newly identified GBX1 gene is expressed in K562 cells and Daudi cells.

【 授权许可】

Unknown