Two different promoters direct expression of two distinct forms of mRNAs of human platelet‐activating factor receptor

FEBS Letters

Terano, Akira¹  Kurachi, Yoshihisa⁴  Honda, Zenichiro²  Izumi, Takashi²  Minami, Michiko²  Mutoh, Hiroyuki²  Bito, Haruhiko²  Nakata, Ryo³  Shimizu, Takao²  Nakamura, Motonao² 

[1] Department of Second Internal Medicine, Faculty of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113, Japan;Department of Biochemistry, Faculty of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113, Japan;The Institute for Adult Diseases, Asahi Life Foundation, Rochester, MN 55905, USA;Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, Mayo Foundation, Rochester, MN 55905, USA

关键词: Lipid mediator;    Alternative splicing;    Primer extension;    Initiator (Inr);    Human leukocyte;    PAF;    platelet activating factor;    Inr;    initiator;    EoL-1 cells;    human eosinophilic cell line;    GM-CSF;    granulocyte macrophage colony stimulating factor;    IL-5;    interleukin-5;    HL-60 cells;    human promyelocytic leukemia cell line;    SSC;    standard saline citrate;    BSA;    bovine serum albumin;    M-MuLV;    Moloney murine leukemia virus;    RACE;    rapid amplification of cDNA ends;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    TdT;    terminal deoxynucleotidyltransferase;   

DOI  :  10.1016/0014-5793(93)81552-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human platelet-activating factor (PAF) receptor gene exists as a single copy on chromosome 1. We identified two 5′-noncoding exons, each of which has distinct transcriptional initiation sites. These exons are alternatively spliced to a common splice acceptor site on a third exon that contains the total open reading frame to yield two different species of functional mRNA (Transcript 1 and 2). Transcript 1 has consensus sequences for transcription factor NF-κB and Sp-1, and the Initiator (Inr) sequence homologous to the murine terminal deoxynucleotidyltransferase gene. Transcript 2 also contains consensus sequences for transcription factor AP-1, AP-2, and Sp-1. Transcripts 1 and 2 were both detected in heart, lung, spleen, and kidney, whereas only Transcript 1 was found in peripheral leukocytes, a differentiated human eosinophilic cell line (EoL-1 cells), and brain. Existence of distinct promoters was thus suggested to play a role in the regulatory control of PAF receptor gene expression in different human tissues and cells.

【 授权许可】

Unknown