Interaction of urokinase‐type plasminogenactivator (u‐PA) with its cellular receptor (u‐PAR) induces phosphorylation on tyrosine of a 38 kDa protein

FEBS Letters

Petri, T.¹  Schleuning, W.-D.¹  Dumler, I.¹ 

[1] Research Laboratories of Schering AG, D-1000 Berlin 65, Germany

关键词: Plasminogen activator;    Urokinase;    Urokinase-type plasminogen activator receptor;    Tyrosine phosphorylation;    U-937 cell;    Signal transduction;    u-PA;    urokinase-type plasminogen activator;    u-PAR;    u-PA receptor;    PMA;    phorbol 12-myristate 13-acetate;    DFP;    diisopropyl fluorophosphate;    DSS;    disuccinimidyl suberate;    PBS;    phosphate buffered saline;    SDS;    sodium dodecyl sulphate;    PAGE;    polyacrylamide gel electrophoresis;    DTT;    dithiothreitol;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(93)81106-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We demonstrate by immunoprecipitation that u-PAR is associated with a 38 kDa protein that is phosphorylated on tyrosine after u-PA treatment of cells. As tyrosine phosphorylation is the hallmark of many signal transduction pathways that promote growth and differentiation, these data suggest that u-PA, besides its role as a regulatory protease, might act as a para- or autocrine hormone.

【 授权许可】

Unknown