The ZNF35 human zinc finger gene encodes a sequence‐specific DNA‐binding protein

FEBS Letters

Cannada-Bartoli, Paola¹  Lania, Luigi¹  Pengue, Gina¹ 

[1] Dipartimento di Genetica, Biologia Generale e Molecolare, University of Naples, Via Mezzocannone 8, 80134 Naples, Italy

关键词: DNA-binding protein;    Zinc finger protein;    DNA-binding site;   

DOI  :  10.1016/0014-5793(93)80115-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We developed a rapid method to determine DNA-binding sites for putative DNA-binding proteins. This procedure has been successfully used to define a specific consensus site for the human ZNF35 zinc finger gene. ZNF35 encodes a 58-kDA polypeptide containing 11 consecutive finger motifs located at the amino terminus, and an acidic domain located at the carboxy terminus. These features suggest that ZNF35 is a site-specific DNA-binding protein involved in the regulation of gene expression. We have expressed the ZNF35 protein from E. coli and have employed a Southwestem-polymerase chain reaction method using random oligonucleotides to identify its high-affinity binding site. The core sequence for the ZNF35 protein-binding site is 5'-C/GC/GAAG/TA-3'.

【 授权许可】

Unknown