Prostaglandin H synthase

FEBS Letters

Miroshnikov, Konstantin A.¹  Igumnova, Natalia D.¹  Mevkh, Alevtina T.¹  Varfolomeev, Sergey D.¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow, Russian Federation

关键词: Prostaglandin H synthase;    Diethyl pyrocarbonate;    Inactivation in the course of the reaction;    Chemical modification;   

DOI  :  10.1016/0014-5793(93)80109-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prostaglandin H synthase (PGHS) as apo-PGHS, holo-PGHS, and holo-PGHS, inactivated in the course of catalysis was studied using chemical modification with diethyl pyrocarbonate (DEPC). The exhausted reaction with DEPC corresponded to the modification of 7 histidine residues in apo-PGHS and 4 in holo-PGHS. All 18 histidine residues became accessible for modification with DEPC in the enzyme, inactivated in the course of catalysis. The velocities of tryptic cleavage of all the three forms into two fragments were fairly different but independent of modification. Based on the results we hypothesize fast and dramatic changes in the protein structure in the course of the substrate conversion.

【 授权许可】

Unknown