Supercoil‐stabilized left‐handed DNA in the plasmid (dA‐dT)16 insert formed in the presence of Ni2+

FEBS Letters

Nejedlý, K.²  Paleček, E.²  Kłysik, J.¹ 

[1] Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Boczna 5, 90-362 Łódź, Poland;Institute of Biophysics, Czechoslovak Academy of Sciences, Královopolská 135, 612 65 Brno, Czechoslovakia

关键词: Supercoiled DNA;    (dA-dT)16 tract structure;    Chemical modification;    Diethyl pyrocarbonate;    Nuclease Bal31;   

DOI  :  10.1016/0014-5793(89)80152-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The (dA-dT)₁₆ insert of the plasmid pAT32 was probed with diethyl pyrocarbonate (DEPC) and nuclease Bal31 in the presence of Ni²⁺ known to be able to induce transition to left-handed conformation in the synthetic poly(dA-dT)·poly (dA-T). It has been shown that this insert in a supercoiled plasmid displays a DEPC modification pattern characteristic of left-handed DNA under conditions not sufficient to induce a left-handed structure in the linear plasmid and poly(dA-dT)·poly(dA-T).

【 授权许可】

Unknown