Divergent effects of fluoroaluminates on the peptide chain elongation factors EF‐Tu and EF‐G as members of the GTPase superfamily

FEBS Letters

Kraal, Barend¹  Mesters, Jeroen R.¹  de Graaf, J.Martien¹ 

[1] Department of Biochemistry, Leiden University, 2300 RA Leiden, The Netherlands

关键词: Guanine-nucleotide binding centre;    γ-Phosphate analogue;    GTPase effector;    G-protein;    Translational inhibition;   

DOI  :  10.1016/0014-5793(93)80097-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fluoroaluminates are thought to mimic the γ-phosphate of GTP and thus, together with GDP, perturb the functioning of heterotrimeric GTP-binding G-proteins. Here we show they do inhibit the ribosome-stimulated GTPase activity of EF-G from Escherichia coli via the formation of a stable complex with EF-G·GDP and ribosomes. In contrast, no perturbed interactions were observed in a similar ribosomal complex with EF-Tu. Interestingly, in the absence of ribosomes both EF-Tu and EF-G remain totally unaffected by fluoroaluminates. For members of the GTPase superfamily such differential effects have not been described before.

【 授权许可】

Unknown