Excitotoxin lesion of nucleus basalis causes a specific decrease in Go mRNA in cerebral cortex

FEBS Letters

Wood, Helen¹  de Belleroche, Jacquline¹ 

[1] Department of Biochemistry, Charing Cross and Westminster Medical School, Fulham Palace Road, London, W6 8RF, UK

关键词: G-protein;    Nucleus basalis;    Cerebral cortex;    Messenger RNA;    MK-801;    W-methyl-D-aspartate receptor;   

DOI  :  10.1016/0014-5793(90)81051-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lesions of the ascending cholinergic pathway from nucleus basalis are known to have profound effects on cortical function. In particular, a substantial potentiation of carbachol-stimulated polyphosphoinositide turnover is detected from 1 day after lesion and is maintained for several days before returning to normal by 1 month. In this study the effect of this lesion was investigated on levels of three G-protein α-subunit mRNAs. Excitotoxin lesion of the nucleus basalis caused a selective reduction in the levels of G_oα mRNA in cerebral cortex ipsilateral to the lesion, G₅α and G_iα mRNA being unaffected. The maximal effect was obtained at 3 days after lesion where levels of G_oα mRNA were decreased by 40% compared to sham-operated animals. Levels of G_oα mRNA returned to normal values by 28 days. Treatment with MK-801 caused a significant attenuation of the decrease in G_oα mRNA, indicating the involvement of NMDA receptors in this response.

【 授权许可】

Unknown