Endothelin‐converting enzyme activity in human serum lipoprotein fraction

FEBS Letters

Hirata, Yukio²  Sakai, Hiroshi¹  Ohwaki, Tatsuya¹ 

[1] Pharmaceutical Research Center, Nisshin Flour Milling Co. Ltd., 5-3-1 Tsurugaoka, Ohimachi, Iruma-gun, Saitama 354, Japan;Second Department of Internal Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113, Japan

关键词: Endothelin;    Lipoprotein;    Endothelin converting enzyme;    ET-1;    endothelin-1;    big ET-1;    big endothelin-1;    IR-ET-1;    immunoreactive endothelin-1;    ECE;    endothelin converting enzyme;   

DOI  :  10.1016/0014-5793(93)80084-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endothelin-1 (ET-1)-converting enzyme (ECE) activity in the human serum lipoprotein fraction was studied using a sensitive enzyme immunoassay and reverse-phase high performance liquid chromatography. The ECE activity of cleaving synthetic human big ET-1 into ET-1 by the serum lipoprotein fraction was about 14-times greater than that by whole serum, and the activity was closely associated with lipoprotein itself. The lipoprotein ECE activity, which was optimal at pH 7.0, was inhibited by EDTA, o-phenanthroline, phosphoramidon, thiorphan, phenylmethane-sulfonyl fluoride and chymostatin, but not by cysteine or aspartic proteinase inhibitors, suggesting metalloproteinase- and chymotrypsin-like properties. These results suggest that the serum lipoprotein ECE may be involved in the processing of big ET-1 to ET-1 in the circulatory system.

【 授权许可】

Unknown