GPER is required for the age-dependent upregulation of the myocardial endothelin system

LIFE SCIENCES

卷:159

Article; Proceedings Paper

Meyer, Matthias R.¹  Fredette, Natalie C.¹  Sharma, Geetanjali¹  Barton, Matthias²  Prossnitz, Eric R.¹ 

[1] Univ New Mexico, Hlth Sci Ctr, Dept Internal Med, 2325 Camino Salud NE, Albuquerque, NM 87131 USA

[2] Univ Zurich, Mol Internal Med, Zurich, Switzerland

关键词: Aging;    Endothelin converting enzyme;    Endothelin receptor;    Estrogen receptor;    ET-1;    GPR30;    Heart;    Myocardium;   

DOI  :  10.1016/j.lfs.2016.02.041

来源: Elsevier

【 摘 要 】

Aims: Cardiac aging is associated with progressive structural changes and functional impairment, such as left ventricular hypertrophy, fibrosis and diastolic dysfunction. Aging also increases myocardial activity of endothelin-1 (ET-1), a multifunctional peptide with growth-promoting and pro-fibrotic activity. Because the G protein coupled estrogen receptor (GPER) regulates vascular responsiveness to ET-1, we investigated whether GPER also plays a role in the regulation of the myocardial endothelin system with aging. Main methods: Young (4 month-old) and aged (24 month-old) wild-type and Gper-deficient (Gper(-/-)) mice were studied. Gene expression levels of prepro-ET-1, endothelin converting enzymes ECE-1 and ECE-2, and endothelin ETA and ETB receptors were determined by qPCR in left ventricular myocardium. Key findings: Aging markedly increased steady-state mRNA expression levels of ECE-1, ECE-2, ETA and ETB receptors (each p < 0.001 vs. young mice). Deletion of Gper inhibited the age-dependent increase in ECE-2 and ETB receptor mRNA levels (57% and 40% reduction, respectively, each p < 0.01 vs. wild-type mice), whereas gene expression of prepro-ET-1, ECE-1, and the ETA receptor was unaffected in Gper(-/-) mice. Significance: We identified a novel regulatory mechanism through which the endogenous Gper facilitates the age dependent increase in myocardial expression of ECE-2 and the ETB receptor, which is compatible with an activating role of GPER for the local endothelin system with aging. Targeting GPER signaling by selective antagonists may therefore be considered a new therapeutic approach to reduce age-dependent increased ET-1 activity and the associated development of left ventricular hypertrophy, fibrosis and heart failure. (C) 2016 Elsevier Inc. All rights reserved.

【 授权许可】

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