Molecular cloning of a mouse myosin I expressed in brain

FEBS Letters

Jiang, Xin¹  Koslovsky, Janet S.¹  Qian, Chungeng¹  Mercer, John A.¹ 

[1] Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75235-9040, USA

关键词: Myosin heavy chain;    Cytoskeleton;    Cell motility;   

DOI  :  10.1016/0014-5793(93)80075-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated two cDNAs that encode putative myosin I heavy chains by polymerase chain reaction amplification of brain cDNA with degenerate oligodeoxynucleotide primers representing myosin I-speeific conserved amino acid sequences. We report the complete deduced amino acid sequence of one of these cDNAs. The sequence is most similar to those of the avian and bovine brush border myosin Is, with five putative calmodulin-binding repeats at the head-tail junction. Northern analysis demonstrates that this myosin heavy chain, unlike the brush border myosins, is expressed in many tissues.

【 授权许可】

Unknown