cDNA encoding the chicken ortholog of the mouse dilute gene product Sequence comparison reveals a myosin I subfamily with conserved C‐terminal domains

FEBS Letters

Kilimann, Manfred W.¹  Meyer, Helmut E.¹  Lichte, Beate¹  Sanders, Gabriele¹ 

[1] Institut für Physiologische Chemie, Ruhr-Universität Bochum, Postfach 102148, W-4630 Bochum 1, Germany

关键词: Dilute;    Myosin heavy chain;    Glutamic acid decarboxylase;    Cytoskeleton;    Vesicle transport;    Synapse;   

DOI  :  10.1016/0014-5793(92)81123-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the cDNA-deduced primary structure of the chicken counterpart of the murine dilute gene product, a member of the myosin I family. Comparison of the chicken and mouse sequences reveals a distinct pattern of domains of high and low sequence conservation. An internal deletion of 25 amino acids probably reflects differential mRNA processing. Compared with other myosin heavy chain molecules, sequence similarity is highest with the MYO2 gene product of Saccharomyces cerevisiae. The MYO2 protein, implicated in vectorial vesicle transport, is homologous to the dilute protein over practically its entire length. In addition, the C-terminal domain of the dilute protein is highly similar to a putative glutamic acid decarboxylase sequence cloned from mouse brain. Alternatively, this closely related clone might represent an isoform of the dilute protein derived from a second gene, potentially involved in genetic conditions related to dilute.

【 授权许可】

Unknown