Participation of the overproduced elongation factor Tu from Thermus thermophilus in protein biosynthesis of Escherichia coli

FEBS Letters

Sprinzl, Mathias¹  Zeidler, Waltraud¹  Kreutzer, Roland¹ 

[1] Laboratorium für Biochemie, Universität Bayreuth, Universitätsstraße 30, 8580 Bayreuth, Germany

关键词: Kirromycin;    tuf-gene expression;    Polysome block;    GTPase activity;   

DOI  :  10.1016/0014-5793(93)80064-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of the overproduced elongation factor Tu (EF-Tu) from Thermus thermophilus on the protein biosynthesis in Escherichia coli was investigated both in vivo and in vitro. A kirromycin-resistant E. coli strain became sensitive to this antibiotic upon the expression of the tufA-gene of T. thermophilus present on a plasmid. In in vitro translation with components of the kirromycin-resistant E. coli strain the poly(Phe) synthesis stopped when minute amounts of the EF-Tu from T. thermophilus were added. Both results indicate the sensitivity of the T. thermophilus EF-Tu to kirromycin and its participation in the polypeptide synthesis of E. coli.

【 授权许可】

Unknown