Archaebacterial elongation factor Tu insensitive to pulvomycin and kirromycin

FEBS Letters

Nicolaus, Barbara¹  Gambacorta, Agata¹  Chinali, Gianni³  Cammarano, Piero²  Teichner, Angela²  de Rosa, Mario¹  Londei, Paola² 

[1] Istituto per La Chimica e Fisica di Molecole di Interesse Biologico, CNR, via Toiano 2, Arco Felice, Naples, Italy;Istituto di Biologia Generale, Facoltà di Medicina, Università di Roma, Policlinico Umberto I, Viale Regina Elena, 00161 Rome, Italy;Cattedra di Chimica e Propedeutica Biochimica, II Facoltà Medica, Università di Napoli, Italy

关键词: Archaebacteria;    Elongation factor Tu;    Pulvomycin;    Kirromycin;    DTT;    dithiothreitol;    aa-tRNA;    aminoacyl-tRNA;    EF;    elongation factor;   

DOI  :  10.1016/0014-5793(82)80819-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A spermine-dependent, polyphenylalanine-synthesizing cell-free system having an optimum activity at 75–85°C, has been developed from the extremely thermoacidophilic archaebacterium Caldariella acidophila. The C. acidophila system is totally insensitive to the EF-Tu targeted antibiotics pulvomycin (at 40°C) and kirromycin (at 47–72°C) contrary to control systems derived from both mesophilic (Escherichia coli) and thermoacidophilic (Bacillus acidocaldarius) eubacteria. The archaebacterial EF-Tu-equivalent factor is also immunologically unrelated to eubacterial EF-Tu and does not cross react with antibodies against Escherichia coli EF-Tu. The pulvomycin and kirromycin reactions thus provide new phyletic markers for archaebacterial ancestry.

【 授权许可】

Unknown