Rainbow trout ovarian cholesterol side‐chain cleavage cytochrome P450 (P450scc)

FEBS Letters

Sakai, Noriyoshi¹  Nagahama, Yoshitaka¹  Miller, Walter L.²  Takahashi, Mika³  Adachi, Shinji¹  Tanaka, Minoru¹ 

[1] Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444, Japan;Department of Pediatrics and the Metabolic Research Unit, University of California, San Francisco, CA 94143, USA;Department of Molecular Biomechanics, The Graduate University for Advanced Studies, Okazaki 444, Japan

关键词: Rainbow trout;    Cytochrome P450scc;    Expression in COS-1 cells;    Steroidogenesis;   

DOI  :  10.1016/0014-5793(93)80034-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with that of human, and 46% homology with that of rat, bovine and pig. P450scc activity was confirmed by transfected COS-1 monkey kidney tumour cells with an expression vector for trout P450scc cDNA and subsequent detection of conversion from 25-hydroxycholesterol to pregnenolone by radioimmunoassay. The cDNA only hybridized to a single 1.8 kb RNA transcript. The transcript was not found in early vitellogenic follicles, barely detected in postvitellogenic follicles, and abundant in postovulatory follicles.

【 授权许可】

Unknown