Isolation and characterization of a high molecular weight type IV collagenase isolated from human carcinoma tissue

FEBS Letters

Yamamura, Masaichi²  Matsuzaki, Shohei¹  Tsuda, Tomi T.²  Kodama, Akira³  Tsuda, Michio² 

[1]Internal Medicine, School of Medicine, Tokai University, Isehara, Kanagawa 259-11, Japan

[2]Molecular Life Science, School of Medicine, Tokai University, Isehara, Kanagawa 259-11, Japan

[3]BML Inc., R&D Center, Saitama 350, Japan

关键词: High molecular weight type IV collagenase;    Type IV collagenase;    Metastatic carcinoma tissue;    APMA;    4-aminophenylmercuric acetate;    DTT;    dithiothreitol;    ECM;    extracellular matrix;    EDTA;    ethylenediaminetetraacetic acid;    HPLC;    high performance liquid chromatography;    MMPs;    matrix metalloproteinases;    PAGE;    polyacrylamide gel electrophoresis;    PMSF;    phenylmethylsulfonyl fluoride;    SDS;    sodium dodecyl sulfate;    TIMP;    tissue inhibitor of metalloproteinases;   

DOI  :  10.1016/0014-5793(93)80032-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A proform of high molecular weight type IV collagenase was isolated and purified 1230-fold from human metastatic carcinoma tissue. Like matrix metalloproteinases (MMPs), the enzyme was activated by trypsin and degraded type IV collagen and gelatin at a neutral pH, the activity was inhibited by EDTA and o-phenanthroline. However, the molecular weight was much higher than MMPs which degraded type IV collagen, gelatinase A (MMP-2; 72 kDa gelatinase/type IV collagenase) (EC 3.4.24.24), gelatinase B (MMP-9; 92 kDa gelatinase/type IV collagenase) (EC 3.4.24.35), stromelysin-1 (MMP-3; 57 kDa) (EC 3.4.24.17) and stromelysin-2 (MMP-10; 57 kDa) (EC 3.4.24.22). The other significant difference from MMPs was that the enzyme was not activated by 4-aminophenylmercuric acetate nor inhibited by TIMP. Taking together these results, this high molecular weight type IV collagenase might be a newly found enzyme different from MMPs or might have the same configuration as MMPs already reported.

【 授权许可】

Unknown