A novel TIMP‐insensitive type IV collagen‐degrading metalloproteinase from murine metastatic sarcoma cells

FEBS Letters

Hayakawa, Taro²  Yamamoto, Shuji¹  Kato, Yoichi¹  Abe, Shigeto³  Ogawa, Kohei¹  Kishi, Jun-ichi² 

[1] Bio-Science Laboratory, Life Science Research Laboratories, Asahi Chemical Industry Co. Ltd, 2-1 Samejima, Fuji-City, Shizuoka 416, Japan;Department of Biochemistry, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464, Japan;Rehabilitation Center, Miyagino Hospital (Sendai), 4-16-1 Higashisendai, Miyagino-ku, Sendai 983, Japan

关键词: Type IV collagenase;    Metastasis;    TIMP (tissue inhibitor of metalloproteinase);    Basement membrane;    Metalloproteinase;    Mouse T241 sarcoma cell;    TIMP;    tissue inhibitor of metalloproteinases;    APMA;    4-aminophenylmercuric acetate;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    HPLC;    high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(90)80967-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme degrades type IV collagen, but neither type I collagen nor casein. The failure of trypsin treatment to enhance the enzyme activity suggested that the purified enzyme did not require activation. Although the enzyme seems to be classified as a matrix metalloproteinase, it was inhibited by neither tissue inhibitor of metalloproteinases (TIMP) nor TIMP-2 and thus represents a novel type IV collagen-degrading metalloproteinase.

【 授权许可】

Unknown