The circulating bioactive form of human guanylin is a high molecular weight peptide (10.3 kDa)

FEBS Letters

Adermann, Knut²  Forssmann, Wolf-Georg²  Kuhn, Michaela²  Raida, Manfred²  Schulz-Knappe, Peter²  Gerzer, Rupert¹  Heim, Jörg-Martin³ 

[1] DLR Institut für Flugmedizin, Linder Höhe, 5000 Köln 90, Germany;Niedersächsisches Institut für Peptid-Forschung (IPF), Feodor-Lynen Str. 31, 3000 Hannover 61, Germany;Labor für Klinische Pharmakologie, Medizinische Klinik Innenstadt der Universität, Ziemssenstraße 1, 8000 München 2, Germany

关键词: Guanylin;    Guanylate cyclase;    Cyclic GMP;    Peptide hormone;    Hemofiltrate;   

DOI  :  10.1016/0014-5793(93)80022-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Guanylin is a peptide isolated from rat intestine that stimulates intestinal guanylate cyclase. We describe here the purification of circulating guanylin from human hemofiltrate. By N-terminal protein sequence analysis 47 amino acids were determined. This sequence corresponds to the positions 22 to 68 of the prohormone deduced from the cDNA sequence of human proguanylin. Mass spectral analysis of the circulating peptide showed the molecular weight to be 10,336 Da, which corresponds to the mass calculated from position 22 to the C-terminus of the peptide predicted from the cDNA sequence. Circulating guanylin markedly increased the cyclic GMP content of T84 cells. Our data show that the hormonal form of guanylin is circulating as a 10.3-kDa peptide in human blood.

【 授权许可】

Unknown