Human guanylin: cDNA isolation, structure, and activity

FEBS Letters

Kato, Johji¹  Currie, Mark G.¹  Huang, Margaret D.³  Wiegand, Roger C.³  Kachur, James F.⁴  Fok, Kam F.² 

[1] Departments of Molecular Pharmacology, Monsanto Corporate Research, Monsanto Company, St. Louis, MO 63198, USA;Departments of Chemistry, Monsanto Corporate Research, Monsanto Company, St. Louis, MO 63198, USA;Departments of Molecular Biology, Monsanto Corporate Research, Monsanto Company, St. Louis, MO 63198, USA;Department of Immunoinflammatory Diseases Research, G.D. Searle, Skokie, IL, USA

关键词: Guanylin;    Heat-stable enterotoxin;    Guanylate cyclase;    Cyclic GMP;   

DOI  :  10.1016/0014-5793(92)81387-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Guanylin is a mammalian peptide homologue of heat-stable enterotoxins that acts on intestinal guanylate cyclase to elicit an increase in cyclic GMP. We have isolated a cDNA encoding an apparent precursor of guanylin from a human intestinal cDNA library. The mRNA is expressed at high levels in human ileum and colon. Human guanylin stimulated increases in T84 cell cyclic GMP levels, displaced ¹²⁵I-labelled heat-stable enterotoxin (STa) binding to this cell line, and stimulated increases in short-circuit current (Isc) or isolated rat proximal colonic mucosa. This peptide may play a role in regulating fluid and electrolyte absorption in human intestines.

【 授权许可】

Unknown