Identification of domains of a cloned rat brain GABA transporter which are not required for its functional expression

FEBS Letters

Bendahan, Annie¹  Kanner, Baruch I.¹ 

[1] Department of Biochemistry, Hadassah Medical School, The Hebrew University, P.O. Box 1172, Jerusalem, 91010, Israel

关键词: GABA transport;    Truncated cDNA clone;    Expression in HeLa cells;    Vaccinia/T7 recombinant virus;    Rat brain;   

DOI  :  10.1016/0014-5793(93)81323-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sodium and chloride coupled γ-aminobutyric acid (GABA) transporter purified from rat brain, belongs to a superfamily of neurotransmitter transporters. They are involved in the termination of synaptic transmission and are predicted to have 12 membrane spanning α-helices with both amino- and carboxyl-termini oriented toward the cytoplasm. In order to define the domains not required for functional expression, we have constructed and expressed a series of deletion mutants in GAT-1, the cDNA clone encoding for the transporter. Transporters truncated at either end until just a few amino-acids distance from the beginning of helix 1 and the end of helix 12, retain their ability to catalyze sodium and chloride-dependent GABA transport.

【 授权许可】

Unknown