Neuronal and glial γ‐aminobutyric acid+ transporters are distinct proteins

FEBS Letters

Kanner, Baruch I.¹  Rauen, Thomas²  Frese, Michael²  Mabjeesh, Nicola J.¹  Jeserich, Gunnar² 

[1] Department of Biochemistry, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem, Israel;Department of Animal Physiology, University of Osnabruck, W4500 Osnabruck, Germany

关键词: Astroglial cell;    Membrane vesicle;    GABA transport;    Immunofluorescence;    Sequence directed antibody;    Immunoblotting;    GABA;    γ-aminobutyric acid;    ACHC;    cis-3-aminacyclohexane carboxylic acid;    GFAP;    glial fibrillary acid protein;   

DOI  :  10.1016/0014-5793(92)80109-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the central nervous system, two subtypes of sodium- and chloride-coupled GABA transporter exist. One is sensitive to ACHC, the other to β-alanine. They are thought to be of neuronal and glial origin, respectively. GABA transport in membrane vesicles derived from astroglial cells was found to be sodium- and chloride-dependent, electrogenic and much more sensitive to β-alanine than to ACHC. Immunoblotting with antibodies directed against a variety of sequences of the ACHC-sensitive transporter indicated that none of these epitopes was shared by the glial transporter.

【 授权许可】

Unknown