Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

FEBS Letters

Waugh, Robbie²  Kulesza, Helen¹  Brown, John W.S.²  Beggs, Jean D.²  Simpson, Gordon G.² 

[1] Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK;Cellular and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK

关键词: Pre-mRNA splicing;    PRP8;    snRNP;    Spliceosomal protein;    Tobacco;    Affinity-purified antibodies;   

DOI  :  10.1016/0014-5793(93)81315-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.

【 授权许可】

Unknown