期刊论文详细信息
FEBS Letters | |
Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura‐2 | |
Voges, Dieter1  Huber, Robert1  Berendes, Robert1  Burger, Alexander1  Demange, Pascal1  | |
[1] Max-Planck-Institut für Biochemie, Am Klopferspitz, 8033 Martinsried, Germany | |
关键词: Annexin V; Calcium channel; Liposome; Fura-2; EGTA; ethylene glycol (bis(2-aminoethyl ether)) N; N; N'; N'-tetraacetic acid; EDTA; ethylene diamine tetraacetic acid; LUV; large unilamellar vesicles; MLV; multilamellar vesicles; TMA; tetramethylammonium hydroxide; | |
DOI : 10.1016/0014-5793(93)81507-V | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.
【 授权许可】
Unknown
【 预 览 】
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