Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura‐2

FEBS Letters

Voges, Dieter¹  Huber, Robert¹  Berendes, Robert¹  Burger, Alexander¹  Demange, Pascal¹ 

[1] Max-Planck-Institut für Biochemie, Am Klopferspitz, 8033 Martinsried, Germany

关键词: Annexin V;    Calcium channel;    Liposome;    Fura-2;    EGTA;    ethylene glycol (bis(2-aminoethyl ether)) N;    N;    N';    N'-tetraacetic acid;    EDTA;    ethylene diamine tetraacetic acid;    LUV;    large unilamellar vesicles;    MLV;    multilamellar vesicles;    TMA;    tetramethylammonium hydroxide;   

DOI  :  10.1016/0014-5793(93)81507-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.

【 授权许可】

Unknown