FEBS Letters | |
Rat platelets are deficient in internal Ca2+ release and require influx of extracellular Ca2+ for activation | |
Feijge, Marion A.H.1  Heemskerk, Johan W.M.1  Hornstra, Gerard1  Rietman, Ellen1  | |
[1] Departments of Biochemistry and Human Biology, Biomedical Center, University of Limburg, P.O. Box 616, 6200 MD Maastricht, The Netherlands | |
关键词: ADP; Calcium channel; Fura-2; Platelet; Thrombin; (Rat); | |
DOI : 10.1016/0014-5793(91)80690-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Calcium fluxes were studied in fura-2-labeled rat platelets. Thrombin, ADP and ionomycin induced rapid mobilization of internally stored Ca2+, which resulted in only a moderate increase of cytosolic [Ca2+]i. Thrombin and ADP stimulated influx of extracellular Ca2+, which was monitored as uptake of 45Ca2+ and of Mn2+. With either agonist, the influx of Ca2+ magnified the initial increase of [Ca2+]i. Since responses of rat platelets were dependent on external [Ca2+], we conclude that Ca2+ influx complements the mobilization of internal stores to reach sufficiently high [Ca2+]i for full activation. A regulatory effect of protein kinase C modulators was observed on both agonist-induced elevation of [Ca2+]i and receptor-mediated Ca2+ entry.
【 授权许可】
Unknown
【 预 览 】
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