Induction of metallothionein in a human astrocytoma cell line by interleukin‐1 and heavy metals

FEBS Letters

Kikuchi, Yutaka²  Terao, Tadao³  Kasahara, Tadashi¹  Sawada, Jun-ichi³  Irie, Masachika² 

[1] Department of Medical Biology and Parasitology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi, Kawachi, Tochigi 329-04, Japan;Department of Microbiology, Hoshi College of Pharmacy, 2-4-41 Ebara, Shinagawa, Tokyo 142, Japan;Division of Biochemistry and Immunochemistry, National Institute of Hygienic Sciences, 1-18-1 Kamiyoga. Setagaya, Tokyo 158, Japan

关键词: Metallothionein;    Interleukin-1;    Interleukin-6;    Heavy metal;    Astrocytoma cell;    U373MG cell;   

DOI  :  10.1016/0014-5793(93)81483-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of cytokines and heavy metals on the expression and localization of metallothioneins (MTs) within U373MG astrocytoma cells were analyzed by using indirect immunofluorescence using a monoclonal anti-MT antibody (MT45). IL-1, CdCl₂ (50μM) or ZnCI₂, (500μM) remarkably augmented intracellular MT levels, whereas IL-6 or 10 μM of ZnCl₂ showed no inducing activity. From 24 to 48 h after the addition of CdCl₂ or IL-1, immunoreactive MTs were found in the cytoplasm and the nucleus. After 72 h, immunoreactive MTs accumulated in a granular form near the cell surfaces in the presence of CdCl₂ (50 μM) or IL-1 plus ZnCI₂ (10 μM). However, this accumulation was not observed when only IL-1 was added. Thus, Zn²⁺ facilitated the appearance of the granular form of immunoreactive MTs at a concentration where they do not induce MTs by themselves.

【 授权许可】

Unknown