Effects of tyrosine kinase inhibitors on protein kinase‐independent systems

FEBS Letters

Tavaré, Jeremy M.¹  Denton, Richard M.¹  Hudson, Alan T.²  Young, Stephen W.¹  Halestrap, Andrew P.¹  Poole, Robert C.¹ 

[1] Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK;The Wellcome Research Laboratories, Langley Court, South Eden Park Road, Beckenham, Kent, BR3 3BS, UK

关键词: Tyrosine kinase;    Inhibitor;    Insulin;    Fat cell;    Lactate transport;    Cell growth;    HEPES;    4-(2-hydroxyethyl)-1-piperazineethanesul-phonic acid;    MOPS;    morpholinopropane sulphonic acid;    EGF;    epidermal growth factor;    PDGF;    platelet-derived growth factor;    TMPD;    N;    N;    N;    ‘N’.-tetramethyl-p-phenylene-diamine;    FCCP;    carbonyl cyanide p-(trifluoromethoxy)phenyl hydrazone.;   

DOI  :  10.1016/0014-5793(93)81308-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tyrosine kinase inhibitors have been widely used to probe the role of tyrosine phosphorylation in cellular signalling. These inhibitors exhibit an apparent specificity for tyrosine kinases over the serine/threonine kinases but little is known about their effects on other enzymes or biological systems. We demonstrate that genistein, erbstatin and α-cyanoeinnamamides (tyrphostins) have inhibitory effects on fatty acid synthesis, lactate transport, mitochondrial oxidative phosphorylation and aldehyde dehydrogenase. We propose, therefore, that results obtained using tyrosine kinase inhibitors should be interpreted with caution, particularly if used at concentrations sufficient to inhibit these non-protein kinase-dependent events.

【 授权许可】

Unknown