Adenosine analogs inhibit the guanine‐7‐methylation of mRNA cap structures

FEBS Letters

Bu, Guojun¹  Sitz, Thomas O.¹  Oxenrider, Keith A.¹ 

[1] Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA

关键词: Guanine-7-methyltransferase;    mRNA methylation;    Cap;    Ethionine;    Neplanocin A;    3-Deazaneplanocin A;    SAH;    S-adenosylhomocysteine;    SAM;    S-adeno-sylmethionine;    SAE;    S-adenosylethionine;    NPC;    neplanocin A;    deazaNPC;    3-deazaneplanocin A.;   

DOI  :  10.1016/0014-5793(93)81307-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The adenosine analogs neplanocin A and deazaneplanocin A were observed to inhibit the in vivo guanine-7-methylation of mRNA cap structure using a new assay for hypomethylated RNA. Treatment of cultured mammalian cells with these adenosine analogs resulted in the same extent of hypomethylation of cap structure as did ethionine injection in mice. Neplanocin A and its non-metabolizable analog 3-deazaneplanocin A show the same maximal level of inhibition of methylation suggesting that these adenosine analogs exert their effects by elevating S-adenosylhomocysteine levels rather than by conversion to other inhibitory compounds.

【 授权许可】

Unknown