Effect of ethionine on the in vitro synthesis and degradation of mitochondrial translation products in yeast

FEBS Letters

Téllez, Rowena¹  Jacob, Germaine¹  Basilio, Carlos¹  George-Nascimento, Carlos¹ 

[1] Department of Biochemistry and Nutrition, University of Puerto Rico School of Medicine, San Juan 00936, Puerto Rico

关键词: Mitochondria;    Ethionine;    Degradation;    ATP stimulation;    Mitochondrial translation product;   

DOI  :  10.1016/0014-5793(85)80049-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of ethionine, an amino acid analog of methionine, has been studied in Saccharomyces cerevisiae in relation to cell growth, oxygen consumption, in vitro protein synthesis of mitochondrial translation products (MTPs) and the degradation of those mitoribosomally made proteins by an ATP-dependent process present within the organelle. Ethionine was found to increase the generation time of those cells already committed to cell division and to abolish the initiation of new cell cycles. Oxygen consumption of cultures grown in the presence of the analog was drastically reduced. Ethionine was also found to impair the incorporation of methionine and leucine into mitochondrial translation products, however the synthesis of proteins was not totally blocked and, apparently, mitochondria utilized ethionine as a precursor amino acid. MTPs synthesized by isolated mitochondria in the presence of ethionine were rapidly degraded inside the organelle at a faster rate compared with the normal proteins synthesized under identical conditions in the mitochondria. It is also shown that these in vitro synthesized proteins are degraded by an ATP-stimulated proteolytic system, as has been previously established.

【 授权许可】

Unknown