Molecular weight‐determination of biosynthetically modified monomeric and oligomeric muropeptides from Escherichia coli by plasma desorption‐mass spectrometry

FEBS Letters

Schmid, Erich R.¹  de Pedro, Miguel A.²  Pittenauer, Ernst¹  Allmaier, Günter¹  Caparrós, Marta² 

[1] Institute for Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090, Vienna, Austria;Centra de Biología Molecular, Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049, Madrid, Spain

关键词: High performance liquid chromatography;    Plasma desorption-mass spectrometry;    Molecular weight determination;    d-Amino acid;    Peptidoglycan;   

DOI  :  10.1016/0014-5793(93)81211-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The presence of certain d-amino acids in the growth media of Escherichia coli results in the accumulation of 2 major and 3–5 minor new muropeptides in the murein sacculus. Preliminary data suggested that the major muropeptides correspond to a monomer and a cross-linked dimer with one residue of d-amino acid per molecule. We have analyzed several d-amino acid-modified muropeptides by plasma desorption-mass spectrometry. Our results confirmed that the general structures of the major modified muropeptides are: GlucNAc-MurNAc-l-Ala-d-Glu-m-A2pm-d-X, and GlucNAc-MurNAc-l-Ala-d-Glu-m-A2pm-d-Ala; GlucNAc-MurNAc-l-Ala-d-Glu-m-A2pm-d-X, being X a residue of the d-amino acid. These results corroborate the usefulness of this technique for the structural analysis of muropeptides.

【 授权许可】

Unknown