FEBS Letters | |
Molecular weight‐determination of biosynthetically modified monomeric and oligomeric muropeptides from Escherichia coli by plasma desorption‐mass spectrometry | |
Schmid, Erich R.1  de Pedro, Miguel A.2  Pittenauer, Ernst1  Allmaier, Günter1  Caparrós, Marta2  | |
[1] Institute for Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090, Vienna, Austria;Centra de Biología Molecular, Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049, Madrid, Spain | |
关键词: High performance liquid chromatography; Plasma desorption-mass spectrometry; Molecular weight determination; d-Amino acid; Peptidoglycan; | |
DOI : 10.1016/0014-5793(93)81211-H | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The presence of certain d-amino acids in the growth media of Escherichia coli results in the accumulation of 2 major and 3–5 minor new muropeptides in the murein sacculus. Preliminary data suggested that the major muropeptides correspond to a monomer and a cross-linked dimer with one residue of d-amino acid per molecule. We have analyzed several d-amino acid-modified muropeptides by plasma desorption-mass spectrometry. Our results confirmed that the general structures of the major modified muropeptides are: GlucNAc-MurNAc-l-Ala-d-Glu-m-A2pm-d-X, and GlucNAc-MurNAc-l-Ala-d-Glu-m-A2pm-d-Ala; GlucNAc-MurNAc-l-Ala-d-Glu-m-A2pm-d-X, being X a residue of the d-amino acid. These results corroborate the usefulness of this technique for the structural analysis of muropeptides.
【 授权许可】
Unknown
【 预 览 】
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