cDNA cloning and expression of an Arabidopsis GTP‐binding protein of the ARF family

FEBS Letters

Regad, Farid²  Bardet, Claude²  Lescure, Bernard²  Moisan, Annick¹  Tremousaygue, Dominique²  Axelos, Michèle² 

[1] Station de Biométrie, GDR-1003 CNRS, Centre INRA d'Auzeville, F-31326 Castanet-Tolosan, France;Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, GDR-1003 CNRS, Centre INRA d'Auzeville, F-31326 Castanet-Tolosan, France

关键词: ADP-ribosylation factor;    Small GTP-binding protein;    Suspension cell culture;    Arabidopsis thaliana;   

DOI  :  10.1016/0014-5793(93)81201-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone encoding a small GTP-binding protein, the ADP-ribosylation factor (ARF) was isolated from a cDNA library of Arabidopsis thaliana cultured cells. The predicted amino acid sequence was highly homologous to the known yeast, bovine and human ARF sequences. Southern analysis of Arabidopsis genomic DNA suggested the existence of at least two copies of ARF genes. The level of ARF mRNA was found to be nearly constant during all cell growth stages in suspension cultures.

【 授权许可】

Unknown