Early induction of Na+‐dependent uridine uptake in the regenerating rat liver

FEBS Letters

Casado, F.Javier¹  Felipe, Antonio¹  Ruiz-Montasell, Bonaventura¹  Martinez-Mas, Joan Vicenç¹  Pastor-Anglada, Marçal¹  Enrich, Carlos² 

[1] Unitat de Bioquímica i Biologia Molecular, Departament de Bioquímica i Fisiologia, Universitat de Barcelona, Barcelona, Spain;Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain

关键词: Nucleoside transport;    Liver regeneration;    Rat liver;   

DOI  :  10.1016/0014-5793(93)81741-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Na⁺-dependent uridine transport into liver plasma membrane vesicles from partially hepatectomized and sham-operated rats was studied. Preparations purified 6 h after 70% hepatectomy exhibited an increased V _max of uridine uptake (3.7 vs. 1.4 pmol/mg prot/3 s) without any change in Km (6μM). Incubation of the vesicles in the presence of monensin decreased uridine uptake although the differences between both experimental groups remained identical. It is concluded that uridine transport is induced early after partial hepatectomy by a mechanism which does not involve changes in the transmembrane Na⁺ gradient. This is the first evidence in favor of modulation of nucleoside transport into liver cells.

【 授权许可】

Unknown