Photoaffinity labelling of the nucleoside transporter of cultured mouse lymphoma cells

FEBS Letters

Paterson, Alan R.P.³  Young, James D.¹  Almeida, Anthony F.³  Jarvis, Simon M.² 

[1] Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong;Department of Physiology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7;Cancer Research Group (McEachern Laboratory) Edmonton, Alberta, Canada

关键词: Nucleoside transport;    Photoaffinity labelling;    Cultured mouse lymphoma cell;    Nitrobenzylthioinosine;    Nitrobenzylthioinosine covatent binding;    NBMPR;    6-(4-nitrobenzyl)thio-9-β-D-ribofuranosylpurine (nitrobenzylthioinosine);    NBTGR;    2-amino-6-(4-nitrobenzyl)thio-9-β-D-ribofuranosylpurine (nitrobenzylthioguanosine);    PBS;    Dulbecco's phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(84)81215-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitrobenzylthioniosine (NBMPR), a potent and specific inhibitor of nucleoside transport, is bound reversibly by high affinity sites on nucleoside transporter proteins of erythrocyte membranes and, upon photoactivation, NBMPR molecules become covalently bonded to the sites. This study showed that [³H]NBMPR molecules reversibly bound to intact S49 and L5178Y mouse lymphoma cells became covalently bound upon exposure to UV light. Electrophoretic analysis of plasma membrane fractions from the labelled cells showed that ³H was present in polypeptides which migrated as a major band with an apparent M _r of 45000–65000.

【 授权许可】

Unknown