FEBS Letters | |
Conformational junctions between left‐handed DNA in (dA‐dT)16 and contiguous B‐DNA in a supercoiled plasmid contain chemically reactive bases | |
Lilley, D.M.J.1  Nejedlý, K.2  Paleček, E.2  | |
[1] Department of Biochemistry, The University, Dundee DD1 4HN, UK;Institute of Biophysics, Czechoslovak Academy of Sciences, Královopolská 135, 612 65 Brno, Czechoslovakia | |
关键词: Supercoiled DNA; Left-handed (dA-dT)16; B-Z junction; Unpaired bases; Chemical probe; Bromoacetaldehyde; Chloroacetaldehyde; BAA; bromoacetaldehyde; CAA; Chloroacetaldehyde; DEPC; diethyl pyrocarbonate.; | |
DOI : 10.1016/0014-5793(93)81178-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Alternating adenine-thymine sequences in supercoiled DNA may undergo a transition to the left-handed Z-conformation in the presence of Ni2+ ions and high Na+ concentrations [(1989) FEBS Lett. 243, 313-317]. In this work we have studied the junctions between B- and Z-conformations in a supercoiled plasmid containing a (dA-dT)16 insert, by means of chemical probing. We observed enhanced reactivity of bases at both ends of the alternating tract to chloro- and bromoacetaldehyde. The degree of chemical reactivity was found to increase with the level of negative supercoiling. Only individual bases were observed to be reactive in the B-Z junctions, consistent with tightly localized interfacial regions.
【 授权许可】
Unknown
【 预 览 】
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RO201912020297343ZK.pdf | 608KB | download |