Cyclosporin A suppression of uncoupling in liver mitochondria of ground squirrel during arousal from hibernation

FEBS Letters

Brustovetsky, N.N.¹  Egorova, M.V.¹  Gnutov, D.Yu.¹  Skulachev, V.P.²  Mokhova, E.N.² 

[1] Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russian Federation;A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation

关键词: Thermoregulatory uncoupling;    Cyclosporin A;    Fatty acid;    Hibernation;    BSA;    bovine serum albumin;    CSA;    cyclosporin A;    CAtr;    carboxyatractylate;    EGTA;    ethylene glycol bis(β-aminoethylether)-N;    N'-tetraacetic acid;    FFA;    free fatty acids;    DNP;    2;    4-dinitrophenol;    TPP+;    tetraphenyl phosphonium cation.;   

DOI  :  10.1016/0014-5793(93)81170-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Energy coupling parameters were studied in liver mitochondria of ground squirrel during arousal from hibernation. It was found that such mitochondria become uncoupled during incubation with phosphate in a salt medium. The uncoupling was revealed by respiration rate increase and membrane potential decrease in the presence of oligomycin. Both effects were reversed by addition of cyclosporin A. Under the same in vitro conditions, mitochondria from aroused (active) animals showed no uncoupling but could be uncoupled by addition of palmitate in the cyclosporin A-sensitive fashion. It is proposed that formation of cyclosporin A-sensitive pores can be involved in urgent heat production in arousing hibernators.

【 授权许可】

Unknown