Multiple UDP‐glucuronyltransferases for the glucuronidation of thyroid hormone with preference for 3,3',5'‐triiodothyronine (reverse T3)

FEBS Letters

Visser, Theo J.⁴  van Raaij, Jeroen A.G.M.³  Kaptein, Ellen⁴  Burchell, Brian¹  Ebner, Thomas¹  Joe, Carol Tjong Tjin² 

[1] Department of Biochemical Medicine, University of Dundee, Dundee, Scotland, UK;Department of Clinical Chemistry, School of Pharmacy, University of Utrecht, Utrecht, The Netherlands;TNO Medical Biological Laboratory, Rijswijk, The Netherlands;Department of Internal Medicine III, Erasmus University Medical School, Rotterdam, The Netherlands

关键词: Thyroid hormone;    lodothyronine;    Glucuronidation;    Bilirubin;    Androsterone;    Phenol;    Rat strains;   

DOI  :  10.1016/0014-5793(93)81134-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the glucuronidation of the thyroid hormones T₄, T₃ and rT₃ by liver microsomes of Wistar, Gunn and WAG rats. Gunn rats have a defect in the gene coding for bilirubin and phenol UDP-glucuronyltransferase (UGT) isoenzymes; WAG rats have a genetic defect in androsterone UGT. In normal Wistar rats UGT activity was ≈5-fold higher for rT₃ than for T₄ or T₃. UGT activities for T₄ and rT₃, but not for T₃, were impaired in Gunn rats. Conversely, UGT activity for T₃, but not for T₄ or rT₃, was impaired in WAG rats. Thus, in rat liver rT₃ is glucuronidated much more rapidly than T₄ and T₃. Our results support the view that T₄ and rT₃ are glucuronidated by bilirubin and phenol UGTs and T₃ by androsterone UGT.

【 授权许可】

Unknown