In vitro glucuronidation of xanthohumol, a flavonoid in hop and beer, by rat and human liver microsomes

FEBS Letters

Yilmazer, M.²  Buhler, D.R.²  Stevens, J.F.¹ 

[1] Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA;Department of Environmental and Molecular Toxicology, ALS Building 1007, Oregon State University, Corvallis, OR 97331, USA

关键词: Xanthohumol;    Flavonoid;    Hop;    Glucuronidation;    Rat;    Human;    XN;    xanthohumol;    IX;    isoxanthohumol;    PB;    phenobarbital;    ISF;    isosafrole;    BNF;    β-naphthoflavone;    G1;    glucuronide 1;    G2;    glucuronide 2;    G3;    glucuronide 3;    G4;    glucuronide 4;    HPLC;    high-performance liquid chromatography;    LC/MS;    liquid chromatography/mass spectrometry;    1H-NMR;    proton nuclear magnetic resonance;    UDPGA;    uridine 5′-diphosphoglucuronic acid;   

DOI  :  10.1016/S0014-5793(01)02210-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Xanthohumol (XN) is the major prenylated flavonoid of hop plants and has been detected in beer. Previous studies suggest a variety of potential cancer chemopreventive effects for XN, but there is no information on its metabolism. The aim of this study was to investigate in vitro glucuronidation of XN by rat and human liver microsomes. Using high-performance liquid chromatography, two major glucuronides of XN were found with either rat or human liver microsomes. Release of the aglycone by enzymatic hydrolysis with β-glucuronidase followed by liquid chromatography/mass spectrometry and nuclear magnetic resonance analysis revealed that these were C-4′ and C-4 monoglucuronides of XN.

【 授权许可】

Unknown