Selective cleavage of 28S rRNA variable regions V3 and V13 in myeloid leukemia cell apoptosis

FEBS Letters

Døskeland, Stein O.¹  Houge, Gunnar²  Lanotte, Michel²  Bøe, Roald¹ 

[1] Department of Cell Biology and Anatomy, University of Bergen, Årstadv. 19, N-5009 Bergen, Norway;INSERM U-301, SDINo 15954.1 CNRS, Centre Hayem, Hôpital St. Louis F-75010 Paris, France

关键词: Myeloid leukemia;    Apoptosis;    28S ribosomal RNA;   

DOI  :  10.1016/0014-5793(93)81123-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vigorous apoptosis is induced 3–4 hours after activation of cAMP-dependent protein kinase I in the rat myeloid leukemia cell line IPC-81 [J. Cell. Physiol. 146 (1991) 73-80]. We will report a novel feature of apoptosis in these cells: a selective and temporarily ordered cleavage within the two largest 28S ribosomal RNA variable regions (V3 and V13). The cleavage of 28S rRNA coincided with internucleosomal DNA fragmentation and cessation of cellular protein synthesis. The implication of 28S variable regions as targets in apoptosis is a clue to the function of these so far apparently superfluous parts of eukaryotic ribosomes.

【 授权许可】

Unknown