Human urokinase contains Ga1NAcβ(1‐4)[Fucα(1‐3)]G1cNAcβ(1‐2) as a novel terminal element in N‐linked carbohydrate chains

FEBS Letters

van Oostrum, Jan¹  Vliegenthart, Johannes F.G.²  Bergweff, Aldert A.²  Thomas-Oates, Jane E.³  Kamerling, Johannis P.² 

[1] Ciba-Geigy Ltd., Department of Biotechnology, Basel, Switzerland;Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands;Bijvoet Center, Department of Mass Spectrometry, Utrecht University, Utrecht, The Netherlands

关键词: Urokinase;    Plasminogen activator;    N-Linked carbohydrate chain;    Fast atom bombardment mass spectroscopy (FAD-MS);    1H NMR spectroscopy;    u-PA;    urinary type plasminogen activator;    t-PA;    tissue-type plasminogen activator;    EGF;    epidermal growth factor;    Fuc;    fucose;    Neu5Ac;    N-acetylneuraminic acid;    CHO;    Chinese hamster ovary;    PNGase-F;    peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F;    n.O.e;    nuclear Overhauser enhancement;    HOHAHA;    homonuclear Hartmann-Hahn;    MLEV;    composite pulse devised by Malcom Levitt;    NOESY;    nuclear Overhauser enhancement spectroscopy;    FAB-MS;    fast atom bombardment mass spectrometry;    GLC;    gas liquid chromatography;    FPLC;    fast protein liquid chromatography;    HPLC;    high-pressure liquid chromatography;    Le x;    Lewis x;   

DOI  :  10.1016/0014-5793(92)81512-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Structural analysis of enzymically released N-linked carbohydrate chains of human urokinase (urinary-type plasminogen activator) by ¹H NMR spectroscopy and FAB-MS demonstrated that the N-linked oligosaccharides on the only N-glycosylation site contain diantennary structures with the novel Ga1NAcβ(1-4)[Fucα(1-3)]G1cNAcβ(1-2) element in the upper or the lower branch.

【 授权许可】

Unknown